(10) This single substitution was sufficient to abolish all detectable cleavage of the gp160 envelope precursor polypeptide as well as virus infectivity.(9) After virus release the major portion of precursors is assembled within an approximately 25 nm thick layer directly attached to the envelope.(8) The enzyme was removed from the cell envelope by treatment of the whole cells with sodium dodecyl sulfate, Triton X-100, sodium deoxycholate, and proteinase K. (7) Cells infected with enveloped viruses are good systems for studying both aspects of protein glycosylation, since they contain a limited number of different glycoproteins, often with well-defined functions.(6) The influence of exogenous gangliosides on the structure of the viral envelope was studied using fluorescent and photoactivatable phospholipids incorporated into the viral membrane.(5) Studies using serum from mice that had been immunized with synthetic peptides from the HIV envelope region suggested that this response is directed, at least in part, at several determinants of the transmembrane portion of the HIV envelope glycoprotein.(4) In addition, transitional macrophages with both positive granules and positive RER, nuclear envelope, negative Golgi apparatus (as in exudate- resident macrophages in vivo), and mature macrophages with peroxidatic activity only in the RER and nuclear envelope (as in resident macrophages in vivo) were found.(3) Thus, although ferric-enterochelin cannot penetrate the cell surface from outside, the complex that is formed within the envelope is transported normally into the cell.(2) Sequence variation in the gp116 component of cytomegalovirus envelope glycoprotein B was examined in 11 clinical strains and compared with variation in gp55.(1) The oral nerve endings of the palate, the buccal mucosa and the periodontal ligament of the cat canine were characterized by the presence of a cellular envelope which is the final form of the Henle sheath.
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